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A shortcut method for sexing juvenile European sea bass, Dicentrarchus labrax L. ArchiMer
Menu, Bruno; Peruzzi, Stefano; Vergnet, Alain; Vidal, Marie-odile; Chatain, Beatrice.
The objective of this study was to develop a rapid and effective method of sexing juvenile sea bass with minimum labour and material. To this end, the gonad squash mount technique was applied along with macroscopic techniques for sexing a large number of experimental fish at the age of 215-275 days post fertilization (p.f.). At this age, 90% of the 3894 fish could be unambiguously sexed by macroscopic examination of their gonads, whereas the remaining proportion was identified using squash mount preparations (10%). The accuracy of the observations was measured up to classical histology procedures. Undifferentiated fish accounted for 0.1% of the total population only. The application of the squash mount technique holds out the prospect of considerable...
Tipo: Text Palavras-chave: Histology; Squash; Juvenile; Gonads; Seabass; Dicentrarchus labrax.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-378.pdf
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Heritabilities and GxE interactions for growth in the European sea bass (Dicentrarchus labrax L.) using a marker-based pedigree ArchiMer
Dupont Nivet, M; Vandeputte, Marc; Vergnet, Alain; Merdy, O; Haffray, P; Chavanne, H; Chatain, Beatrice.
253 full-sib families from 33 males and 23 females of European seabass were produced in a partly factorial mating design. All fish were reared in the same tank during 14 months, then 7000 of them were dispatched in four farms to different locations (France, Israel, Italy, Portugal) representing a wide variety of environmental conditions. Around 400 g mean weight, 1177 to 1667 fish in each site were weighed and length was measured. Condition factor (K) was calculated. Pedigrees were redrawn a posteriori using microsatellites markers: parental origin could be retraced for 99.2% of fish. Due to a high incidence of deformities, the useful sample size was reduced to 491-670 fish per site. Maternal effects were small. Using a simple animal model, heritability...
Tipo: Text Palavras-chave: Microsatellites; GxE interactions; Heritability; Growth; Seabass; Dicentrarchus labrax.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4404.pdf
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Response to domestication and selection for growth in the European sea bass (Dicentrarchus labrax) in separate and mixed tanks ArchiMer
Vandeputte, Marc; Dupont Nivet, M; Haffray, P; Chavanne, H; Cenadelli, S; Parati, K; Vidal, Marie-odile; Vergnet, Alain; Chatain, Beatrice.
Selective breeding of European sea bass (Dicentrarchus labrax) receives a growing interest, as the estimated heritability of growth is medium to high. In this study, we compared the offspring of four groups of sea bass sires, mated with the same wild dams: wild (W), first generation of domestication (D), first generation of mass selection for length (M), first generation of PROSPER-like selection for length (P). The comparison was done both in replicated tanks (separate rearing) and in mixed tanks (mixed rearing) where sire origins were recovered by genotyping of eight microsatellite markers. Weight, length and growth rate were measured from day 238 post-fertilization (69 g mean weight) to day 611 post-fertilization (390 g mean weight). Both in mixed and...
Tipo: Text Palavras-chave: Nephrocalcinosis; Competition; Microsatellites; Parentage assignment; Realized heritability; Selection response; Growth; Selective breeding; Dicentrarchus labrax; European sea bass.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-5303.pdf
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What is the heritable component of spinal deformities in the European sea bass (Dicentrarchus labrax)? ArchiMer
Bardon, Agnes; Vandeputte, Marc; Dupont-nivet, Mathilde; Chavanne, Herve; Haffray, Pierrick; Vergnet, Alain; Chatain, Beatrice.
Malformations in farmed fish are a prime research topic as it has a major impact on fish welfare and on the economical value of the product. In this study, the genetic component of spine deformities was estimated in European sea bass, a major species of Mediterranean aquaculture, with the use of a posteriori reconstruction of pedigree with microsatellites. Our population exhibited a very high malformation rate (81%),probably due to intense swimming of fish during pre-growing The heritability of global spine deformities was evaluated to be 0.21 +/- 0.04 on the underlying scale (033 +/- 0.06 for lordosis, 0.13 +/- 0.04 for scoliosis). Deformities scored by an internal or external examination turned out to be genetically the same trait (genetic...
Tipo: Text Palavras-chave: Genetic correlations; Heritability; Growth; Deformities; Dicentrarchus labrax; European sea bass.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6768.pdf
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A first insight into genotype-diet interactions in European seabass (Dicentrarchus labrax) in the context of plant-based diet use ArchiMer
Le Boucher, Richard; Vandeputte, Marc; Dupont Nivet, Mathilde; Quillet, Edwige; Mazurais, David; Robin, Jean; Vergnet, Alain; Medale, Francoise; Kaushik, Sadasivam; Chatain, Beatrice.
Tipo: Text
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00038/14907/12233.pdf
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Evaluation of behavioral changes induced by a first step of domestication or selection for growth in the European sea bass (Dicentrarchus labrax): A self-feeding approach under repeated acute stress ArchiMer
Millot, Sandie; Pean, Samuel; Leguay, Didier; Vergnet, Alain; Chatain, Beatrice; Begout, Marie-laure.
Among other strategies to improve fish welfare in rearing environment, domestication and/or selective breeding was proposed to minimize fish responsiveness to husbandry practices. To verify this hypothesis on a recently domesticated specie, the sea bass, Dicentrarchus labrax, L., an experiment was realized, using four populations differing according to their level of domestication or selection: one population produced from wild parents (Wild), one population produced from parents domesticated for one generation (Domesticated) and two produced from parents selected for growth for one generation (Selected A and Selected B). The experiment was carried out over 91 days with 600 fish (50 fish per tank, 150 fish per population). After a control period, the fish...
Tipo: Text Palavras-chave: Feed intake; Feeding rhythm; Specific growth rate; Adaptation capacities; Welfare.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00011/12254/9030.pdf
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Genotype x environment interactions for growth rate in European sea bass (Dicentrarchus labrax) ArchiMer
Vandeputte, Marc; Dupont-nivet, Mathilde; Haffray, P; Vergnet, Alain; Cenadellii, S; Parati, K; Chavanne, Herve; Laureau, S; Chatain, Beatrice.
Tipo: Text
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00014/12520/9378.pdf
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In search for indirect criteria to improve residual feed intake in sea bass (Dicentrarchus labrax) Part I: Phenotypic relationship between residual feed intake and body weight variations during feed deprivation and re-feeding periods ArchiMer
Grima, Laure; Vandeputte, Marc; Ruelle, Francois; Vergnet, Alain; Mambrini, Muriel; Chatain, Beatrice.
No commercial breeding programs have yet started to improve feed utilization efficiency in fish, mainly because of the difficulty in accurately measuring individual feed intake in fish reared in groups. Our general goal is to propose indirect criteria to be used for breeding of commercial lines. In a previous study undertaken with rainbow trout clones, a genetic correlation was detected between residual feed intake (RFI), and body weight variation during successive periods of feed deprivation (FD) and re-feeding (RF). To assess the pertinence of such indirect criteria for future breeding programs, we set up a large experiment using sea bass (Dicentrachus labrax), a recently domesticated species with broad genetic diversity. The objectives of the present...
Tipo: Text Palavras-chave: European sea bass; Dicentrachus labrax; Feed efficiency; Residual feed intake; Feed deprivation; Compensatory growth; Selective breeding.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11297/7838.pdf
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In search for indirect criteria to improve feed utilization efficiency in sea bass (Dicentrarchus labrax) Part II: Heritability of weight loss during feed deprivation and weight gain during re-feeding periods ArchiMer
Grima, Laure; Chatain, Beatrice; Ruelle, Francois; Vergnet, Alain; Launay, Amandine; Mambrini, Muriel; Vandeputte, Marc.
Selective breeding to improve residual feed intake (RFI) in sea bass (Dicentrarchus labrax) is a major goal that would optimize economic gain while minimizing the environmental impact of production. Due to the difficulty in accurately measuring individual feed intake, no selective breeding program has yet been started. In a previous study, we identified a criterion phenotypically related to RFI variations: the loss of weight during feed deprivation, FD. Moreover, an additional composite criterion (CC) integrating both FD and weight gain during subsequent re-feeding (RF) was closely related to RFI, even though the relationship was only close to significance (P=0.06). The aim of the present study was to estimate the heritability of these two traits, in order...
Tipo: Text Palavras-chave: European sea bass; Dicentrarchus labrax; Feed efficiency; Heritability; Genetic parameters; Residual feed intake; Feed deprivation; Compensatory growth; Selective breeding.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00004/11529/8213.pdf
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Genotype by environment interactions for growth in European seabass (Dicentrarchus labrax) are large when growth rate rather than weight is considered ArchiMer
Dupont-nivet, Mathilde; Karahan-nomm, Bilge; Vergnet, Alain; Merdy, Olivier; Haffray, Pierrick; Chavanne, Herve; Chatain, Beatrice; Vandeputte, Marc.
Two hundred fifty three full-sib families from 33 males and 23 females of European seabass were produced in a partly factorial mating design. All fish were reared in the same tank for 14 months until reaching mean weight of 35 g. then 7000 of them were individually tagged and weighed, and dispatched to four farms in different locations (France, Israel, Italy and Portugal) representing a wide variety of environmental conditions. Around mean weight of 400 g. 1177 to 1667 fish at each site were weighed. Daily growth coefficient (DGC) was calculated. Pedigrees were successfully redrawn for 99.2% of fish using microsatellite markers. Genetic correlations between sites were high for body weight (>0.80 in all cases but one, i.e., five cases over six), but only...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Seabass; Growth; Daily growth coefficient; Heritability; G x E interactions; Aquaculture.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00011/12256/9227.pdf
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A first insight into genotype x diet interactions in European sea bass (Dicentrarchus labrax L. 1756) in the context of plant-based diet use ArchiMer
Le Boucher, Richard; Vandeputte, Marc; Dupont-nivet, Mathilde; Quillet, Edwige; Mazurais, David; Robin, Jean; Vergnet, Alain; Medale, Francoise; Kaushik, Sadasivam; Chatain, Beatrice.
This preliminary study assessed genotype x diet interaction in late growth of European sea bass (Dicentrarchus labrax) fed with either a fish meal (FM)- or a fish oil (FO)-based diet (M) or an all-plant-based (PB) diet. A total of 550 fish from 224 families were reared together and tagged. DNA was sampled and microsatellites were used to assign parentage. When fish weight was 192 +/- 54 g, two tanks were fed with M (FM: 100%; FO: 100%) and two others with PB (FM: 0% and FO: 0%). Body weight (BW), fork length (FL) and fillet lipid content (CorrFat) were analysed with a linear model and with REML methodology. We observed no significant differences between groups, but a slightly lower (P=0.03) daily growth coefficient in sea bass fed PB than in those fed M....
Tipo: Text Palavras-chave: Genotype by diet x environment interaction; European sea bass; Plant diet; Genetic correlation.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00032/14335/12374.pdf
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La sélection pour la vitesse de croissance a-t-elle un impact sur la tolérance au stress et la capacité de nage chez le bar ? ArchiMer
Vandeputte, Marc; Porte, Jean-denis; Auperin, Benoît; Dupont Nivet, Mathilde; Vergnet, Alain; Valotaire, Claudiane; Claireaux, Guy; Prunet, Patrick; Chatain, Beatrice.
Tipo: Text
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00099/20976/18598.pdf
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Fitness of early life stages in F1 interspecific hybrids between Dicentrarchus labrax and D. punctatus ArchiMer
Ky, Chin-long; Vergnet, Alain; Molinari, Nicolas; Fauvel, Christian; Bonhomme, Francois.
Inter- and intraspecific crossbreeding experiments were conducted to evaluate the aquaculture potential of hybrids in the genus Dicentrarchus, focusing on fertilisation and hatching success. The experimental design consisted of 24 controlled crosses in which individual and pooled fertilisations were made between wild D. labrax individuals (8 dams and 5 sires originating from West Mediterranean and Atlantic populations) and wild D. punctatus (6 sires). Three experiments were successively performed: (1) dams from the Mediterranean population, individually considered to have good egg quality, (2) dams from Mediterranean population, individually considered to have poor egg quality and (3) crossbreeding using pooled eggs from the Atlantic population. In each...
Tipo: Text Palavras-chave: Sea bass; Hybrid progeny; Offspring quality; Fitness; Dicentrarchus labrax; Dicentrarchus punctatus.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00078/18881/16451.pdf
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Procédures d'échantillonnage pour la conservation des espèces aquacoles dans la Cryobanque Nationale ArchiMer
Vandeputte, Marc; Labbe, Catherine; Suquet, Marc; Fauvel, Christian; Goardon, Lionel; Duclos, Delphine; Danchin-burge, Coralie; Vergnet, Alain; Rault, Paul; Degremont, Lionel; Benabdelmouna, Abdellah; Brizard, Raphael; Haffray, Pierrick.
Tipo: Text
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00098/20975/18597.pdf
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Genotype by diet interactions in European sea bass (Dicentrarchus labrax L.): Nutritional challenge with totally plant-based diets ArchiMer
Le Boucher, Richard; Vandeputte, Marc; Dupont-nivet, M.; Quillet, E.; Ruelle, Francois; Vergnet, Alain; Kaushik, Sadasivam; Allamellou, J. M.; Medale, F.; Chatain, Beatrice.
Aquaculture of carnivorous species has strongly relied on fish meal and fish oil for feed formulation; however, greater replacement by terrestrial plant-based products is occurring now. This rapid change in dietary environment has been a major revolution and has to be taken into consideration in breeding programs. The present study analyzes potential consequences of this nutritional tendency for selective breeding by estimating genetic parameters of BW and growth rates estimated by the thermal growth coefficient (TGC) over different periods with extremely different diets. European sea bass (Dicentrarchus labrax L.) from a factorial cross (1,526 fish) between 25 sires and 9 dams were used to estimate heritabilities and genotype by diet interaction. Starting...
Tipo: Text Palavras-chave: European sea bass; Genetic correlation; Genotype by diet interaction; Genotype by environment interaction; Plant-based diet.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00146/25711/26299.pdf
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Multi-site evaluation of the rearing performances of 5 wild populations of European sea bass (Dicentrarchus labrax) ArchiMer
Vandeputte, Marc; Garouste, Romain; Dupont-nivet, Mathilde; Haffray, Pierrick; Vergnet, Alain; Chavanne, Herve; Laureau, Stanislas; Ron, Tetsuzan Benny; Pagelson, Glen; Mazorra, Carlos; Ricoux, Remi; Marques, Pedro; Gameiro, Marta; Chatain, Beatrice.
Sea bass is a major species in Mediterranean aquaculture, but has a distribution area ranging from North Atlantic to South Mediterranean, with a population structure previously revealed by population genetics. To test the farming performances of wild sea bass populations, we produced a partial diallel cross mating scheme, using sires originating from North Atlantic (NAT), South Atlantic (SAT), West Mediterranean (WEM), North-East Mediterranean (NEM) and South-East Mediterranean (SEM). Fifteen sires per origin were mated in a full-factorial design using artificial fertilization with 9 NAT dams and 17 WEM dams, producing 10 population crosses and 1950 potential full-sib families. All fish were reared together, then tagged at an average weight of 20 g and...
Tipo: Text Palavras-chave: European sea bass; Dicentrarchus labrax; Selective breeding; Growth; Processing yields; Genotype by environment interaction.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00171/28265/26514.pdf
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Early individual electronic identification of sea bass using RFID microtags: A first example of early phenotyping of sex-related growth ArchiMer
Ferrari, Sebastien; Chatain, Beatrice; Cousin, Xavier; Leguay, Didier; Vergnet, Alain; Vidal, Marie-odile; Vandeputte, Marc; Begout, Marie-laure.
Although individual electronic tagging using PIT (Passive Integrated Transponder) tags is well established, it is mainly used for fish > 60 mm in length. Since electronic tagging is an ideal identification method, we used RFID (Radio Frequency Identification) microtags (6 mm in length, 1 mm in diameter, 10 mg in mass) to characterize individual fish from the early stages of their development and throughout their lifecycles. We used sea bass, Dicentrarchus labrax, (105 day-old fish weighing between 100 and 1100 mg) and studied the effects of intra-coelomic tagging in half the population using different endpoints including survival and tag reading, growth over 6 months and swimming responses. Dead fish were counted daily, biometric data were collected at...
Tipo: Text Palavras-chave: Tagging effects; Swimming behavior; Sexual dimorphism; Dicentrarchus labrax.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00175/28645/27072.pdf
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Effect of selection for fasting tolerance on feed intake, growth and feed efficiency in the European sea bass Dicentrarchus labrax ArchiMer
Daule, Sophie; Vandeputte, Marc; Vergnet, Alain; Guinand, Bruno; Grima, Laure; Chatain, Beatrice.
Feed efficiency is a major goal for aquaculture sustainability, and selecting fish to genetically enhance this trait would be highly valuable. However, no selective breeding program specifically targeted to feed efficiency exists for farmed fish, mostly because of the difficulty of measuring individual feed intake. However, a negative phenotypic correlation between feed efficiency and weight loss at fasting has been previously demonstrated in sea bass submitted to feed deprivation (FD). We mated sea bass parents selected for their high (FD+) or low (FD-) weight loss at fasting to produce FD+ and FD- progeny, which were reared in a single tank to avoid common environmental effects. At 8 months of age, 1200 of those fish were submitted to three alternating...
Tipo: Text Palavras-chave: European sea bass; Dicentrarchus labrax; Selective breeding; Feed efficiency; Fasting tolerance.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00189/29985/28483.pdf
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Combining vitassign and colony: an efficient practical procedure for parental assignment with missing parents ArchiMer
Doan, Q.k.; Vandeputte, Marc; Chatain, Beatrice; Vergnet, Alain; Allal, Francois.
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00281/39196/37750.pdf
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Combining vitassign and colony for pedigree reconstruction in a case of factorial mating with missing parental genotypes ArchiMer
Allal, Francois; Doan, Q.k.; Chatain, Beatrice; Vergnet, Alain; Vandeputte, Marc.
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00306/41694/40971.pdf
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